Protein-coding gene in the species Homo sapiens
3-hydroxyisobutyrate dehydrogenase |
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Identifiers |
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EC no. | 1.1.1.31 |
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CAS no. | 9028-39-1 |
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Databases |
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IntEnz | IntEnz view |
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BRENDA | BRENDA entry |
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ExPASy | NiceZyme view |
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KEGG | KEGG entry |
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MetaCyc | metabolic pathway |
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PRIAM | profile |
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PDB structures | RCSB PDB PDBe PDBsum |
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Gene Ontology | AmiGO / QuickGO |
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Search |
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PMC | articles |
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PubMed | articles |
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NCBI | proteins |
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HIBADH |
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![](//upload.wikimedia.org/wikipedia/commons/thumb/3/36/Protein_HIBADH_PDB_2gf2.png/250px-Protein_HIBADH_PDB_2gf2.png) |
Available structures |
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PDB | Ortholog search: PDBe RCSB |
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List of PDB id codes |
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2GF2, 2I9P |
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Identifiers |
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Aliases | HIBADH, Hibadh, 6430402H10Rik, AI265272, NS5ATP1, 3-hydroxyisobutyrate dehydrogenase |
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External IDs | OMIM: 608475; MGI: 1889802; HomoloGene: 15088; GeneCards: HIBADH; OMA:HIBADH - orthologs |
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EC number | 1.1.1.31 |
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Gene location (Human) |
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![Chromosome 7 (human)](//upload.wikimedia.org/wikipedia/commons/thumb/9/99/Ideogram_human_chromosome_7.svg/300px-Ideogram_human_chromosome_7.svg.png) | Chr. | Chromosome 7 (human)[1] |
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| Band | 7p15.2 | Start | 27,525,442 bp[1] |
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End | 27,662,883 bp[1] |
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Gene location (Mouse) |
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![Chromosome 6 (mouse)](//upload.wikimedia.org/wikipedia/commons/thumb/c/c0/Ideogram_house_mouse_chromosome_6.svg/260px-Ideogram_house_mouse_chromosome_6.svg.png) | Chr. | Chromosome 6 (mouse)[2] |
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| Band | 6|6 B3 | Start | 52,523,213 bp[2] |
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End | 52,617,374 bp[2] |
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RNA expression pattern |
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Bgee | Human | Mouse (ortholog) |
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Top expressed in | - myocardium of left ventricle
- cardiac muscle tissue of right atrium
- renal medulla
- right adrenal cortex
- human kidney
- left adrenal gland
- quadriceps femoris muscle
- left adrenal cortex
- vastus lateralis muscle
- deltoid muscle
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| Top expressed in | - right kidney
- brown adipose tissue
- epithelium of stomach
- proximal tubule
- interventricular septum
- lacrimal gland
- myocardium of ventricle
- left lobe of liver
- right ventricle
- digastric muscle
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| More reference expression data |
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BioGPS | ![](//upload.wikimedia.org/wikipedia/commons/thumb/e/e3/PBB_GE_HIBADH_gnf1h05976_at_fs.png/250px-PBB_GE_HIBADH_gnf1h05976_at_fs.png) | More reference expression data |
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Gene ontology |
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Molecular function | - 3-hydroxyisobutyrate dehydrogenase activity
- oxidoreductase activity
- NAD binding
- phosphogluconate dehydrogenase (decarboxylating) activity
- NADP binding
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
| Cellular component | - mitochondrial matrix
- mitochondrion
| Biological process | - valine catabolic process
- branched-chain amino acid catabolic process
| Sources:Amigo / QuickGO |
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Wikidata |
View/Edit Human | View/Edit Mouse |
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In enzymology, a 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) also known as β-hydroxyisobutyrate dehydrogenase or 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH) is an enzyme[5] that in humans is encoded by the HIBADH gene.[6]
3-Hydroxyisobutyrate dehydrogenase catalyzes the chemical reaction:
- 3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
Thus, the two substrates of this enzyme are 3-hydroxy-2-methylpropanoate and NAD+, whereas its 3 products are 2-methyl-3-oxopropanoate, NADH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3-hydroxy-2-methylpropanoate:NAD+ oxidoreductase. This enzyme participates in valine, leucine and isoleucine degradation.
Function
3-hydroxyisobutyrate dehydrogenase is a tetrameric mitochondrial enzyme that catalyzes the NAD+-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[6]
Structural studies
As of late 2007, five structures have been solved for this class of enzymes, with PDB accession codes 1WP4, 2CVZ, 2GF2, 2H78, and 2I9P.
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000106049 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029776 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Robinson WG, Coon MJ (March 1957). "The purification and properties of beta-hydroxyisobutyric dehydrogenase". J. Biol. Chem. 225 (1): 511–21. doi:10.1016/S0021-9258(18)64948-8. PMID 13416257.
- ^ a b "Entrez Gene: HIBADH 3-hydroxyisobutyrate dehydrogenase".
Further reading
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature. 424 (6945): 157–64. Bibcode:2003Natur.424..157H. doi:10.1038/nature01782. PMID 12853948.
- Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology". Science. 300 (5620): 767–72. Bibcode:2003Sci...300..767S. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205.
- Mammalian Gene Collection Program Team, Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National Academy of Sciences. 99 (26): 16899–16903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Sanger Centre T, Washington University Genome Sequencing Cente T (1999). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi:10.1101/gr.8.11.1097. PMID 9847074.
- Hughes GJ, Frutiger S, Paquet N, et al. (1994). "Human liver protein map: update 1993". Electrophoresis. 14 (11): 1216–22. doi:10.1002/elps.11501401181. PMID 8313870. S2CID 33424554.
- Rougraff PM, Paxton R, Kuntz MJ, et al. (1988). "Purification and characterization of 3-hydroxyisobutyrate dehydrogenase from rabbit liver". J. Biol. Chem. 263 (1): 327–31. doi:10.1016/S0021-9258(19)57396-3. PMID 3335502.
- Rougraff PM, Zhang B, Kuntz MJ, et al. (1989). "Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase. Evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases". J. Biol. Chem. 264 (10): 5899–903. doi:10.1016/S0021-9258(18)83634-1. PMID 2647728.
External links
- Human HIBADH genome location and HIBADH gene details page in the UCSC Genome Browser.
- PDBe-KB provides an overview of all the structure information available in the PDB for Human 3-hydroxyisobutyrate dehydrogenase, mitochondrial
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1.1.1: NAD/NADP acceptor | |
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1.1.2: cytochrome acceptor | - D-lactate dehydrogenase (cytochrome)
- D-lactate dehydrogenase (cytochrome c-553)
- Mannitol dehydrogenase (cytochrome)
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1.1.3: oxygen acceptor | |
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1.1.4: disulfide as acceptor | |
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1.1.5: quinone/similar acceptor | |
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1.1.99: other acceptors | |
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Activity | |
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Regulation | |
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