Bicinchoninic acid
![]() | |
Names | |
---|---|
Preferred IUPAC name [2,2′-Biquinoline]-4,4′-dicarboxylic acid | |
Other names Bicinchoninic acid 4,4′-Dicarboxy-2,2′-biquinoline 2,2'-Biquinoline-4,4'-dicarboxylic acid | |
Identifiers | |
CAS Number |
|
3D model (JSmol) |
|
ChEMBL |
|
ChemSpider |
|
ECHA InfoCard | 100.013.628 ![]() |
PubChem CID |
|
UNII |
|
CompTox Dashboard (EPA) |
|
InChI
| |
| |
Properties | |
Chemical formula | C20H12N2O4 |
Molar mass | 344.326 g·mol−1 |
Appearance | Cream colored powder |
Odor | Characteristic odor[vague] |
Melting point | 352 °C (666 °F; 625 K) decomposes |
Solubility in water | Partially soluble in cold water, hot water[vague] |
Hazards | |
Occupational safety and health (OHS/OSH): | |
Main hazards | Will irritate eyes and mucous membranes. |
NFPA 704 (fire diamond) | ![]() 1 |
Safety data sheet (SDS) | External MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). ![]() ![]() ![]() Infobox references |
Bicinchoninic acid (/baɪsɪnkɔːnɪnɪk/) or BCA is a weak acid composed of two carboxylated quinoline rings. It is an organic compound with the formula (C9H5NCO2H)2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.[1][2][3]
Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total concentration of protein in a solution. Bicinchoninic acid is used to detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[4]
References
- ^ Smith, P.K.; Krohn, R.I.; Hermanson, G.T.; Mallia, A.K.; Gartner, F.H.; Provenzano, M.D.; Fujimoto, E.K.; Goeke, N.M.; Olson, B.J.; Klenk, D.C. (1985). "Measurement of Protein Using Bicinchoninic Acid". Analytical Biochemistry. 150 (1): 76–85. doi:10.1016/0003-2697(85)90442-7. PMID 3843705.
- ^ Brown, Rhoderick E.; Jarvis, Kari L.; Hyland, Kristi J. (1989). "Protein measurement using bicinchoninic acid: Elimination of interfering substances". Analytical Biochemistry. 180 (1): 136–139. doi:10.1016/0003-2697(89)90101-2. PMID 2817336.
- ^ Noble, James E.; Bailey, Marc J.A. (2009). "Chapter 8 Quantitation of Protein". Guide to Protein Purification, 2nd Edition. Methods in Enzymology. Vol. 463. pp. 73–95. doi:10.1016/S0076-6879(09)63008-1. ISBN 9780123745361. PMID 19892168.
- ^ Pierce BCA Protein Assay Kit