EDANS

EDANS
Names
Preferred IUPAC name
5-[(2-Aminoethyl)amino]naphthalene-1-sulfonic acid
Other names
EDANS
Identifiers
CAS Number
  • 50402-56-7 checkY
3D model (JSmol)
  • Interactive image
ChemSpider
  • 83355 ☒N
ECHA InfoCard 100.051.415 Edit this at Wikidata
PubChem CID
  • 92329
UNII
  • 3JBY896YZF checkY
CompTox Dashboard (EPA)
  • DTXSID70964660 Edit this at Wikidata
InChI
  • InChI=1S/C12H14N2O3S/c13-7-8-14-11-5-1-4-10-9(11)3-2-6-12(10)18(15,16)17/h1-6,14H,7-8,13H2,(H,15,16,17) ☒N
    Key: SJQRQOKXQKVJGJ-UHFFFAOYSA-N ☒N
  • InChI=1/C12H14N2O3S/c13-7-8-14-11-5-1-4-10-9(11)3-2-6-12(10)18(15,16)17/h1-6,14H,7-8,13H2,(H,15,16,17)
    Key: SJQRQOKXQKVJGJ-UHFFFAOYAN
  • c1cc2c(cccc2S(=O)(=O)O)c(c1)NCCN
Properties
Chemical formula
 C12H14N2O3S
Molar mass 266.32
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references
Chemical compound

EDANS (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid) is a donor for FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL. The combination can be used in enzyme assays. When the two compounds are in close proximity, most of the energy emitted from EDANS will be quenched by DABCYL. However, if the compounds are separated (for example, by substrate cleavage) EDANS will fluoresce, giving an indication of enzyme presence.[1]

See also

  • Dark quencher

References

  1. ^ Guo, Jiubiao; et al. (30 November 2014). "Spectral properties of EDANS-Dabcyl pair [45] and the flowchart of the experimental design". PLOS ONE. 9 (12). doi:10.1371/journal.pone.0114124.g001.


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