EDANS
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Names | |
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Preferred IUPAC name 5-[(2-Aminoethyl)amino]naphthalene-1-sulfonic acid | |
Other names EDANS | |
Identifiers | |
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3D model (JSmol) |
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ChemSpider |
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ECHA InfoCard | 100.051.415 ![]() |
PubChem CID |
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CompTox Dashboard (EPA) |
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InChI
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Properties | |
Chemical formula | C12H14N2O3S |
Molar mass | 266.32 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). ![]() ![]() ![]() Infobox references |
Chemical compound
EDANS (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid) is a donor for FRET-based nucleic acid probes and protease substrates. EDANS is often paired with DABCYL or DABSYL. The combination can be used in enzyme assays. When the two compounds are in close proximity, most of the energy emitted from EDANS will be quenched by DABCYL. However, if the compounds are separated (for example, by substrate cleavage) EDANS will fluoresce, giving an indication of enzyme presence.[1]
See also
- Dark quencher
References
- ^ Guo, Jiubiao; et al. (30 November 2014). "Spectral properties of EDANS-Dabcyl pair [45] and the flowchart of the experimental design". PLOS ONE. 9 (12). doi:10.1371/journal.pone.0114124.g001.
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