Middlebrook 7H10 Agar
Growth medium used to culture Mycobacterium species
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis.[1] It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Composition
Ingredients (g/L)[2]
- Ammonium sulfate, 0.50
- Monopotassium phosphate, 1.50
- Disodium phosphate, 1.50
- Sodium citrate, 0.40
- Magnesium sulfate, 0.025
- Zinc sulfate, 0.001
- Copper sulfate, 0.001
- L-Glutamic acid, 0.50
- Ferric ammonium citrate, 0.04
- Pyridoxine hydrochloride, 0.001
- Biotin, 0.0005
- Malachite green, 0.00025
- Agar, 15.00
- Calcium chloride, 0.0005
Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.
See also
- Lowenstein-Jensen medium
- Middlebrook 7H9 Broth
References
External links
- Middlebrook 7H10 Agar
- Traditional Microbiology
- v
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Growth media / agar plates
Gram positive |
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Gram negative |
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- BAF agar
- Czapek medium
- Dermatophyte test medium
- Malt extract agar
- MMN medium
- Potato dextrose agar
- Sabouraud agar
- YEPD
- YM
- Blood agar
- Chocolate agar
- Columbia blood agar
- Letheen broth
- Lysogeny broth
- Nutrient agar
- Plate count agar
- Trypticase soy agar
- Tryptic soy broth
- Zobell's marine agar