NUTF2

Protein-coding gene in the species Homo sapiens
NUTF2
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

1GY5,%%s1GY6, 5BXQ, 1A2K, 1OUN

Identifiers
AliasesNUTF2, NTF2, PP15, NTF-2, nuclear transport factor 2
External IDsOMIM: 605813; MGI: 3704482; HomoloGene: 110745; GeneCards: NUTF2; OMA:NUTF2 - orthologs
Gene location (Human)
Chromosome 16 (human)
Chr.Chromosome 16 (human)[1]
Chromosome 16 (human)
Genomic location for NUTF2
Genomic location for NUTF2
Band16q22.1Start67,846,923 bp[1]
End67,872,567 bp[1]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • muscle of thigh

  • ascending aorta

  • popliteal artery

  • tibial arteries

  • left coronary artery

  • C1 segment

  • Descending thoracic aorta

  • right coronary artery

  • anterior cingulate cortex

  • stromal cell of endometrium
    n/a
More reference expression data
BioGPS
n/a
Gene ontology
Molecular function
  • protein binding
  • structural constituent of nuclear pore
  • identical protein binding
  • nuclear import signal receptor activity
Cellular component
  • cytoplasm
  • nuclear pore central transport channel
  • nuclear outer membrane
  • intracellular anatomical structure
  • extracellular exosome
  • nucleus
  • nuclear inner membrane
  • nuclear pore
  • nucleoplasm
  • cytosol
  • membrane
  • nuclear membrane
Biological process
  • negative regulation of vascular endothelial growth factor production
  • protein transport
  • positive regulation of protein import into nucleus
  • protein export from nucleus
  • protein import into nucleus
  • mRNA transport
  • protein localization to nuclear pore
  • transport
  • nucleocytoplasmic transport
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

10204

621832

Ensembl

ENSG00000102898

n/a

UniProt

P61970

P61971

RefSeq (mRNA)

NM_005796
NM_001322038
NM_001322039
NM_001322040
NM_001322041

XM_001473957

RefSeq (protein)
NP_001308967
NP_001308968
NP_001308969
NP_001308970
NP_005787

NP_005787.1
NP_001308967.1
NP_001308968.1
NP_001308969.1
NP_001308970.1

NP_080808
NP_001344158
NP_001344159

Location (UCSC)Chr 16: 67.85 – 67.87 Mbn/a
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

Nuclear transport factor 2 is a protein that in humans is encoded by the NUTF2 gene.[4][5][6]

Function

The protein encoded by this gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.[6]

Interactions

NUTF2 has been shown to interact with Nucleoporin 62[4][7] and RAN.[8][9]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000102898 – Ensembl, May 2017
  2. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ a b Paschal BM, Gerace L (Jun 1995). "Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62". J Cell Biol. 129 (4): 925–937. doi:10.1083/jcb.129.4.925. PMC 2120498. PMID 7744965.
  5. ^ Grundmann U, Nerlich C, Rein T, Lottspeich F, Küpper HA (Jul 1988). "Isolation of cDNA coding for the placental protein 15 (PP15)". Nucleic Acids Res. 16 (10): 4721. doi:10.1093/nar/16.10.4721. PMC 336660. PMID 3380696.
  6. ^ a b "Entrez Gene: NUTF2 nuclear transport factor 2".
  7. ^ Hu T, Guan T, Gerace L (Aug 1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC 2120945. PMID 8707840.
  8. ^ Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS (Nov 2004). "Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2". J. Mol. Biol. 344 (2): 303–310. doi:10.1016/j.jmb.2004.09.043. PMID 15522285.
  9. ^ Stewart M, Kent HM, McCoy AJ (Apr 1998). "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran". J. Mol. Biol. 277 (3): 635–646. doi:10.1006/jmbi.1997.1602. PMID 9533885.

Further reading

  • Bukrinsky MI, Haffar OK (1998). "HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr". Mol. Med. 4 (3): 138–43. doi:10.1007/BF03401911. PMC 2230352. PMID 9562972.
  • Stewart M (2001). "Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)". Cell Struct. Funct. 25 (4): 217–225. doi:10.1247/csf.25.217. PMID 11129791.
  • Bohn H, Johannsen R, Kraus W (1981). "[New placental protein (PP15) with immunosuppressive properties (author's transl)]". Arch. Gynecol. 230 (2): 167–72. doi:10.1007/BF02108272. PMID 7458421. S2CID 9104166.
  • Hu T, Guan T, Gerace L (1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC 2120945. PMID 8707840.
  • Bullock TL, Clarkson WD, Kent HM, Stewart M (1996). "The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)". J. Mol. Biol. 260 (3): 422–431. doi:10.1006/jmbi.1996.0411. PMID 8757804.
  • Clarkson WD, Corbett AH, Paschal BM, Kent HM, McCoy AJ, Gerace L, Silver PA, Stewart M (1997). "Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran". J. Mol. Biol. 272 (5): 716–730. doi:10.1006/jmbi.1997.1255. PMID 9368653.
  • Stewart M, Kent HM, McCoy AJ (1998). "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran". J. Mol. Biol. 277 (3): 635–646. doi:10.1006/jmbi.1997.1602. PMID 9533885.
  • Ribbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1999). "NTF2 mediates nuclear import of Ran". EMBO J. 17 (22): 6587–6598. doi:10.1093/emboj/17.22.6587. PMC 1171005. PMID 9822603.
  • Steggerda SM, Black BE, Paschal BM (2000). "Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran". Mol. Biol. Cell. 11 (2): 703–19. doi:10.1091/mbc.11.2.703. PMC 14804. PMID 10679025.
  • Freire J, Covelo G, Sarandeses C, Díaz-Jullien C, Freire M (2001). "Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha". Biochem. Cell Biol. 79 (2): 123–131. doi:10.1139/bcb-79-2-123. PMID 11310559.
  • Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS (2004). "Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2". J. Mol. Biol. 344 (2): 303–310. doi:10.1016/j.jmb.2004.09.043. PMID 15522285.
  • Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  • v
  • t
  • e
  • 1a2k: GDPRAN-NTF2 COMPLEX
    1a2k: GDPRAN-NTF2 COMPLEX
  • 1ar0: NUCLEAR TRANSPORT FACTOR 2 (NTF2) E42K MUTANT
    1ar0: NUCLEAR TRANSPORT FACTOR 2 (NTF2) E42K MUTANT
  • 1ask: NUCLEAR TRANSPORT FACTOR 2 (NTF2) H66A MUTANT
    1ask: NUCLEAR TRANSPORT FACTOR 2 (NTF2) H66A MUTANT
  • 1gy5: D92N,D94N DOUBLE POINT MUTANT OF HUMAN NUCLEAR TRANSPORT FACTOR 2 (NTF2)
    1gy5: D92N,D94N DOUBLE POINT MUTANT OF HUMAN NUCLEAR TRANSPORT FACTOR 2 (NTF2)
  • 1gy6: NTF2 FROM RAT, AMMONIUM SULPHATE CONDITIONS
    1gy6: NTF2 FROM RAT, AMMONIUM SULPHATE CONDITIONS
  • 1jb2: CRYSTAL STRUCTURE OF NTF2 M84E MUTANT
    1jb2: CRYSTAL STRUCTURE OF NTF2 M84E MUTANT
  • 1jb4: CRYSTAL STRUCTURE OF NTF2 M102E MUTANT
    1jb4: CRYSTAL STRUCTURE OF NTF2 M102E MUTANT
  • 1jb5: CRYSTAL STRUCTURE OF NTF2 M118E MUTANT
    1jb5: CRYSTAL STRUCTURE OF NTF2 M118E MUTANT
  • 1oun: CRYSTAL STRUCTURE OF NUCLEAR TRANSPORT FACTOR 2 (NTF2)
    1oun: CRYSTAL STRUCTURE OF NUCLEAR TRANSPORT FACTOR 2 (NTF2)
  • 1qma: NUCLEAR TRANSPORT FACTOR 2 (NTF2) W7A MUTANT
    1qma: NUCLEAR TRANSPORT FACTOR 2 (NTF2) W7A MUTANT
  • 1u5o: Structure of the D23A mutant of the nuclear transport carrier NTF2
    1u5o: Structure of the D23A mutant of the nuclear transport carrier NTF2


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