6,7-Dimetil-8-ribitillumazin sintaza

6,7-dimetil-8-ribitillumazin sintaza
Identifikatori
EC broj 2.5.1.78
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC articles
PubMed articles
NCBI Protein search

6,7-dimetil-8-ribitillumazin sintaza (EC 2.5.1.78, lumazinska sintaza, 6,7-dimetil-8-ribitillumazinska sintaza 2, 6,7-dimetil-8-ribitillumazinska sintaza 1, lumazinska sintaza 2, lumazinska sintaza 1, tip I lumazinska sintaza, tip II lumazinska sintaza, RIB4, MJ0303, RibH, Pbls, MbtLS, RibH1 protein, RibH2 protein, RibH1, RibH2) je enzim sa sistematskim imenom 5-amino-6-(D-ribitilamino)uracil butandiontransferaza.[1][2][3][4][5][6][7][8][9][10][11][12][13] Ovaj enzim katalizuje sledeću hemijsku reakciju

1-dezoksi-L-glicero-tetruloza 4-fosfat + 5-amino-6-(D-ribitilamino)uracil ⇌ {\displaystyle \rightleftharpoons } 6,7-dimetil-8-(D-ribitil)lumazin + 2H2O + fosfat

Ovaj enzim učestvuje u biosintezi riboflavina.

Reference

  1. ↑ Kis, K., Volk, R. and Bacher, A. (1995). „Biosynthesis of riboflavin. Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine synthase”. Biochemistry 34: 2883-2892. PMID 7893702. 
  2. ↑ Garcia-Ramirez, J.J., Santos, M.A. and Revuelta, J.L. (1995). „The Saccharomyces cerevisiae RIB4 gene codes for 6,7-dimethyl-8-ribityllumazine synthase involved in riboflavin biosynthesis. Molecular characterization of the gene and purification of the encoded protein”. J. Biol. Chem. 270: 23801-23807. PMID 7559556. 
  3. ↑ Bacher, A., Fischer, M., Kis, K., Kugelbrey, K., Mörtl, S., Scheuring, J., Weinkauf, S., Eberhardt, S., Schmidt-Bäse, K., Huber, R., Ritsert, K., Cushman, M., Ladenstein, R. (1996). „Biosynthesis of riboflavin: structure and mechanism of lumazine synthase”. Biochem. Soc. Trans. 24: 89-94. PMID 8674771. 
  4. ↑ Mörtl, S., Fischer, M., Richter, G., Tack, J., Weinkauf, S. and Bacher, A. (1996). „Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli”. J. Biol. Chem. 271: 33201-33207. PMID 8969176. 
  5. ↑ Bacher, A., Eberhardt, S., Fischer, M., Mortl, S., Kis, K., Kugelbrey, K., Scheuring, J. and Schott, K. (1997). „Biosynthesis of riboflavin: lumazine synthase and riboflavin synthase”. Methods Enzymol. 280: 389-399. PMID 9211334. 
  6. ↑ Goldbaum, F.A., Velikovsky, C.A., Baldi, P.C., Mortl, S., Bacher, A. and Fossati, C.A. (1999). „The 18-kDa cytoplasmic protein of Brucella species - an antigen useful for diagnosis - is a lumazine synthase”. J. Med. Microbiol. 48: 833-839. PMID 10482294. 
  7. ↑ Jordan, D.B., Bacot, K.O., Carlson, T.J., Kessel, M. and Viitanen, P.V. (1999). „Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase”. J. Biol. Chem. 274: 22114-22121. PMID 10419541. 
  8. ↑ Zhang, X., Meining, W., Fischer, M., Bacher, A. and Ladenstein, R. (2001). „X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 Å resolution: determinants of thermostability revealed from structural comparisons”. J. Mol. Biol. 306: 1099-1114. PMID 11237620. 
  9. ↑ Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J.P., Huber, R. and Bacher, A. (2002). „Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe”. Eur. J. Biochem. 269: 519-526. PMID 11856310. 
  10. ↑ Cushman, M., Yang, D., Gerhardt, S., Huber, R., Fischer, M., Kis, K. and Bacher, A. (2002). „Design, synthesis, and evaluation of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase”. J. Org. Chem. 67: 5807-5816. PMID 12153285. 
  11. ↑ Haase, I., Mortl, S., Kohler, P., Bacher, A. and Fischer, M. (2003). „Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii”. Eur. J. Biochem. 270: 1025-1032. PMID 12603336. 
  12. ↑ Morgunova, E., Meining, W., Illarionov, B., Haase, I., Jin, G., Bacher, A., Cushman, M., Fischer, M. and Ladenstein, R. (2005). „Crystal structure of lumazine synthase from Mycobacterium tuberculosis as a target for rational drug design: binding mode of a new class of purinetrione inhibitors”. Biochemistry 44: 2746-2758. PMID 15723519. 
  13. ↑ Morgunova, E., Saller, S., Haase, I., Cushman, M., Bacher, A., Fischer, M. and Ladenstein, R. (2007). „Lumazine synthase from Candida albicans as an anti-fungal target enzyme: structural and biochemical basis for drug design”. J. Biol. Chem. 282: 17231-17241. PMID 17446177. 

Literatura

  • Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X. 
  • Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036. 
  • Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0. 
  • Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097. 
  • Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X. 
  • Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842. 

Spoljašnje veze

  • MeSH 6,7-dimethyl-8-ribityllumazine+synthase
  • p
  • r
  • u
TemeTipovi
EC1 Oksidoreduktaze/spisak  • EC2 Transferaze/spisak  • EC3 Hidrolaze/spisak  • EC4 Lijaze/spisak  • EC5 Izomeraze/spisak  • EC6 Ligaze/spisak
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6