Endonukleaza specifična za strukturu prepusta 1
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Endonukleaza 1 specifična za flap strukturu | |||||||||||
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![]() PDB prikaz baziran na 1ul1. | |||||||||||
Dostupne strukture | |||||||||||
1U7B, 1UL1, 3Q8K, 3Q8L, 3Q8M, 3UVU | |||||||||||
Identifikatori | |||||||||||
Simboli | FEN1; FEN-1; MF1; RAD2 | ||||||||||
Vanjski ID | OMIM: 600393 MGI: 102779 HomoloGene: 3034 GeneCards: FEN1 Gene | ||||||||||
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Pregled RNK izražavanja | |||||||||||
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podaci | |||||||||||
Ortolozi | |||||||||||
Vrsta | Čovek | Miš | |||||||||
Entrez | 2237 | 14156 | |||||||||
Ensembl | ENSG00000168496 | ENSMUSG00000024742 | |||||||||
UniProt | P39748 | P39749 | |||||||||
Ref. Sekv. (iRNK) | NM_004111 | NM_001271614 | |||||||||
Ref. Sekv. (protein) | NP_004102 | NP_001258543 | |||||||||
Lokacija (UCSC) | Chr 11: 61.56 - 61.56 Mb | Chr 19: 10.2 - 10.2 Mb | |||||||||
PubMed pretraga | [1] | [2] |
Flap endonukleaza 1 je enzim koji je kod ljudi kodiran FEN1 genom.[1][2]
Protein kodiran ovim genom uklanja 5' prepuste tokom DNK popravke i obrađuje 5' krajeve Okazakijevih fragmenta pri sintezi zaostajućeg lanca DNK. Direktna fizička interakcija između tog proteina i AP endonukleaze 1 tokom popravke isecanjem dugog segmenta baza omogućava koordinirani pristup proteina supstratu, čime se supstrat dodaje od jednog enzima do drugog. Ovaj protein je član XPG/RAD2 familije endonukleaza i jedan je od deset proteina koji su esencijalni za bezćelijsku replikaciju DNK. Sekundarna struktura DNK može da inhibira flap obradu na pojedinim trinukleotidnim ponavljanjima u maniru zavisnom od dužine koji skrivaju 5' kraj prepusta, koji je neophodan za vezivanje i presecanje proteinom kodiranim ovim genom. Stoga sekundarna struktura može da onemogući protektivnu funkciju ovog proteina, što dovodi do trinukleotidnih ekspanzija specifičnih za mesto.[2]
Interakcije
Endonukleaza 1 specifična za flap strukturu formia interakcije sa Ciklin-zavisna kinaza 2,[3] EP300,[4] ATP-zavisna helikaza Vernerovog sindroma,[5][6] Heterogeni nuklearni ribonukleoprotein A1,[7] Ciklin A2,[3] PCNA,[3][4][8][9][10][11][12] Protein blum sindroma[5] i APEX1.[11]
Reference
- ↑ Hiraoka LR, Harrington JJ, Gerhard DS, Lieber MR, Hsieh CL (Jul 1995). „Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human”. Genomics 25 (1): 220–5. DOI:10.1016/0888-7543(95)80129-A. PMID 7774922.
- ↑ 2,0 2,1 „Entrez Gene: FEN1 flap structure-specific endonuclease 1”.
- ↑ 3,0 3,1 3,2 Henneke, Ghislaine; Koundrioukoff Stéphane; Hübscher Ulrich (Jul 2003). „Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation”. Oncogene (England) 22 (28): 4301–13. DOI:10.1038/sj.onc.1206606. ISSN 0950-9232. PMID 12853968.
- ↑ 4,0 4,1 Hasan, S; Stucki M; Hassa P O; Imhof R; Gehrig P; Hunziker P; Hübscher U; Hottiger M O (Jun 2001). „Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300”. Mol. Cell (United States) 7 (6): 1221–31. DOI:10.1016/S1097-2765(01)00272-6. ISSN 1097-2765. PMID 11430825.
- ↑ 5,0 5,1 Sharma, Sudha; Sommers Joshua A; Wu Leonard; Bohr Vilhelm A; Hickson Ian D; Brosh Robert M (Mar 2004). „Stimulation of flap endonuclease-1 by the Bloom's syndrome protein”. J. Biol. Chem. (United States) 279 (11): 9847–56. DOI:10.1074/jbc.M309898200. ISSN 0021-9258. PMID 14688284.
- ↑ Brosh, R M; von Kobbe C; Sommers J A; Karmakar P; Opresko P L; Piotrowski J; Dianova I; Dianov G L i dr.. (Oct 2001). „Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity”. EMBO J. (England) 20 (20): 5791–801. DOI:10.1093/emboj/20.20.5791. ISSN 0261-4189. PMC 125684. PMID 11598021.
- ↑ Chai, Qing; Zheng Li; Zhou Mian; Turchi John J; Shen Binghui (Dec 2003). „Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment processing during Okazaki fragment maturation”. Biochemistry (United States) 42 (51): 15045–52. DOI:10.1021/bi035364t. ISSN 0006-2960. PMID 14690413.
- ↑ Jónsson, Z O; Hindges R; Hübscher U (Apr 1998). „Regulation of DNA replication and repair proteins through interaction with the front side of proliferating cell nuclear antigen”. EMBO J. (ENGLAND) 17 (8): 2412–25. DOI:10.1093/emboj/17.8.2412. ISSN 0261-4189. PMC 1170584. PMID 9545252.
- ↑ Gary, R; Ludwig D L; Cornelius H L; MacInnes M A; Park M S (Sep 1997). „The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21”. J. Biol. Chem. (UNITED STATES) 272 (39): 24522–9. DOI:10.1074/jbc.272.39.24522. ISSN 0021-9258. PMID 9305916.
- ↑ Chen, U; Chen S; Saha P; Dutta A (Oct 1996). „p21Cip1/Waf1 disrupts the recruitment of human Fen1 by proliferating-cell nuclear antigen into the DNA replication complex”. Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 93 (21): 11597–602. DOI:10.1073/pnas.93.21.11597. ISSN 0027-8424. PMC 38103. PMID 8876181.
- ↑ 11,0 11,1 Dianova, I I; Bohr V A; Dianov G L (Oct 2001). „Interaction of human AP endonuclease 1 with flap endonuclease 1 and proliferating cell nuclear antigen involved in long-patch base excision repair”. Biochemistry (United States) 40 (42): 12639–44. DOI:10.1021/bi011117i. ISSN 0006-2960. PMID 11601988.
- ↑ Yu, P; Huang B; Shen M; Lau C; Chan E; Michel J; Xiong Y; Payan D G i dr.. (Jan 2001). „p15(PAF), a novel PCNA associated factor with increased expression in tumor tissues”. Oncogene (England) 20 (4): 484–9. DOI:10.1038/sj.onc.1204113. ISSN 0950-9232. PMID 11313979.
Literatura
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- Li X, Li J, Harrington J, et al. (1995). „Lagging strand DNA synthesis at the eukaryotic replication fork involves binding and stimulation of FEN-1 by proliferating cell nuclear antigen.”. J. Biol. Chem. 270 (38): 22109–12. DOI:10.1074/jbc.270.38.22109. PMID 7673186.
- Robins P, Pappin DJ, Wood RD, Lindahl T (1994). „Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coli DNA polymerase I.”. J. Biol. Chem. 269 (46): 28535–8. PMID 7961795.
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- Harrington JJ, Lieber MR (1994). „The characterization of a mammalian DNA structure-specific endonuclease.”. EMBO J. 13 (5): 1235–46. PMC 394933. PMID 8131753.
- Shen B, Nolan JP, Sklar LA, Park MS (1996). „Essential amino acids for substrate binding and catalysis of human flap endonuclease 1.”. J. Biol. Chem. 271 (16): 9173–6. DOI:10.1074/jbc.271.16.9173. PMID 8621570.
- Chen U, Chen S, Saha P, Dutta A (1996). „p21Cip1/Waf1 disrupts the recruitment of human Fen1 by proliferating-cell nuclear antigen into the DNA replication complex.”. Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11597–602. DOI:10.1073/pnas.93.21.11597. PMC 38103. PMID 8876181.
- Warbrick E, Lane DP, Glover DM, Cox LS (1997). „Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair.”. Oncogene 14 (19): 2313–21. DOI:10.1038/sj.onc.1201072. PMID 9178907.
- Klungland A, Lindahl T (1997). „Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).”. EMBO J. 16 (11): 3341–8. DOI:10.1093/emboj/16.11.3341. PMC 1169950. PMID 9214649.
- Gary R, Ludwig DL, Cornelius HL, et al. (1997). „The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21.”. J. Biol. Chem. 272 (39): 24522–9. DOI:10.1074/jbc.272.39.24522. PMID 9305916.
- Stöhr H, Marquardt A, Rivera A, et al. (1998). „A gene map of the Best's vitelliform macular dystrophy region in chromosome 11q12-q13.1.”. Genome Res. 8 (1): 48–56. DOI:10.1101/gr.8.1.48. PMC 310689. PMID 9445487.
- Jónsson ZO, Hindges R, Hübscher U (1998). „Regulation of DNA replication and repair proteins through interaction with the front side of proliferating cell nuclear antigen.”. EMBO J. 17 (8): 2412–25. DOI:10.1093/emboj/17.8.2412. PMC 1170584. PMID 9545252.
- Warbrick E, Heatherington W, Lane DP, Glover DM (1998). „PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain.”. Nucleic Acids Res. 26 (17): 3925–32. DOI:10.1093/nar/26.17.3925. PMC 147798. PMID 9705499.
- Hosfield DJ, Mol CD, Shen B, Tainer JA (1998). „Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity.”. Cell 95 (1): 135–46. DOI:10.1016/S0092-8674(00)81789-4. PMID 9778254.
- Dianov GL, Jensen BR, Kenny MK, Bohr VA (1999). „Replication protein A stimulates proliferating cell nuclear antigen-dependent repair of abasic sites in DNA by human cell extracts.”. Biochemistry 38 (34): 11021–5. DOI:10.1021/bi9908890. PMID 10460157.
- Greene AL, Snipe JR, Gordenin DA, Resnick MA (1999). „Functional analysis of human FEN1 in Saccharomyces cerevisiae and its role in genome stability.”. Hum. Mol. Genet. 8 (12): 2263–73. DOI:10.1093/hmg/8.12.2263. PMID 10545607.
- Matsumoto Y, Kim K, Hurwitz J, et al. (1999). „Reconstitution of proliferating cell nuclear antigen-dependent repair of apurinic/apyrimidinic sites with purified human proteins.”. J. Biol. Chem. 274 (47): 33703–8. DOI:10.1074/jbc.274.47.33703. PMID 10559261.
- Spiro C, Pelletier R, Rolfsmeier ML, et al. (2000). „Inhibition of FEN-1 processing by DNA secondary structure at trinucleotide repeats.”. Mol. Cell 4 (6): 1079–85. DOI:10.1016/S1097-2765(00)80236-1. PMID 10635332.
- Hasan S, Stucki M, Hassa PO, et al. (2001). „Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300.”. Mol. Cell 7 (6): 1221–31. DOI:10.1016/S1097-2765(01)00272-6. PMID 11430825.
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